Name: Jeffrey M. Canfield Home Page: http://ierc.med.uiuc.edu/canfield.html or http://www.biosci.ohio-state.edu/~jcanfld/ Address: 2211 Briarcliff Rd NE #2, Atlanta, GA 30329 Phone: (404) 636-4189 (answering machine there) E-mail: canfield@physics.emory.edu or canfield@engineering.uiuc.edu Date Available: negotiable Degrees: University of Illinois at Urbana-Champaign (UIUC) Ph.D. Physics Graduate GPA: 3.976 of 4.0 Oct. 1997 M.S. Physics Aug. 1991 B.S. Engineering Physics May 1990 GPA: Departmental 4.0, Overall 3.956 of 4.0 Highest Honors and Bronze Tablet 1990 James Scholar 1986-89 Awards: DOE Subcontract via Oak Ridge National Laboratory Summer 1999 Postdoctoral Fellowship-The Ohio State University 1997-98 NIH Traineeship in Radiation Oncology 1994-96 Department of Defense NDSEG Fellowship (USAF AFOSR) 1990-93 Professional Experience: Postdoctoral Research Associate-Emory University 2000-2007 Postdoctoral Researcher-The Ohio State University 1999-2000 Lecturer at The Ohio State University (OSU) General Chemistry for the Sciences II Spring 1999 General Chemistry for Science & Engineering I Winter 1999 Teaching Assistant at UIUC General Physics for the Life Sciences II Spring 1997 Slichter's Principles of Magnetic Resonance Spring 1996 Physical Chemistry (Spectroscopy) Lab Spring 1994 Biomolecular Physics Fall 1993 IBM Summer Intern-Atlanta, GA and Lexington, KY 1989 & 1990 Object-Oriented Programming and Laserprinter Product Research & Development University of North Carolina-Chapel Hill 1988 Undergraduate Summer Research Program in Molecular Biology and Biotechnology Used molecular biology techniques to study a lysosomal protein Hoffer Plastics Corporation-South Elgin, IL 1988 & 1989 Engineering Study Abroad in China-Summer Program 1987 Xian Jiaotong University, Xian, China Studied Chinese language, history, basic industry Model United Nations at UIUC 1986-89 Secretary 1987-88, Treasurer 1988-89 Computer Skills (most self-taught): Very proficient in Matlab, C, UNIX, Applesoft BASIC, and LaTeX Familiar with Fortran, Mathematica, HyperChem, Quanta, Sigma, DOS, VMD, InsightII, TCL, Cobol, Smalltalk Have used Apple, IBM, NeXT, SGI, HP, and Sun computers Languages: Learned oral Chinese (Mandarin) in China Memberships: American Physical Society, American Chemical Society International EPR (ESR) Society Association for Computing Machinery Society for Industrial and Applied Mathematics Publications: (1) "A Perturbation Theory Treatment of Oscillating Magnetic Fields in the Radical Pair Mechanism" by J. M. Canfield, R. L. Belford, P. G. Debrunner, and K. J. Schulten Chemical Physics vol.182 no.1 pp.1-18 Apr.15 1994 Chemical Physics vol.191 no.1-3 p.347 Feb.1 1995 http://dx.doi.org/10.1016/0301-0104(93)E0442-X http://dx.doi.org/10.1016/0301-0104(94)00234-2 (2) "A Perturbation Treatment of Oscillating Magnetic Fields in the Radical Pair Mechanism using the Liouville Equation" by J. M. Canfield, R. L. Belford, P. G. Debrunner, and K. Schulten Chemical Physics vol.195 no.1-3 pp.59-69 Jun.1 1995 http://dx.doi.org/10.1016/0301-0104(95)00049-T (3) "Calculations of Earth-Strength Steady and Oscillating Magnetic Field Effects in Coenzyme B12 Radical Pair Systems" by J. M. Canfield, R. L. Belford, and P. G. Debrunner Molecular Physics vol.89 no.3 pp.889-930 Oct.20 1996 http://search.epnet.com/direct.asp?an=7613123&db=aph (4) "Approaching Magnetic Field Effects in Biology Using the Radical Pair Mechanism" by J. M. Canfield, Doctoral Thesis Department of Physics, University of Illinois at Urbana-Champaign, 1997 UMI (Dissertation Abstracts) control # AAT9812544 (vol.59 no.9 1999) call 1-800-521-3042 from US or 1-800-343-5299 from Canada or see http://www.umi.com/hp/Support/DServices/order/ to order also available at many university libraries (OCLC/WorldCat lists some) (5) "Geometry of Reactant Centers in the CoII-Substrate Radical Pair State of Coenzyme B12-Dependent Ethanolamine Deaminase Determined by Using Orientation-Selection-ESEEM Spectroscopy" by J. M. Canfield and K. Warncke Journal of Physical Chemistry B vol.106 no.34 pp.8831-8841 Aug.29 2002 http://dx.doi.org/10.1021/jp0207634 (6) "Functional Relationship of Serine 90 Phosphorylation and the Surrounding Putative Salt Bridge in Bovine Prolactin" by E. J. H. Schenck, J. M. Canfield, and C. L. Brooks Molecular and Cellular Endocrinology vol.204 no.1-2 pp.117-125 Jun.30 2003 http://dx.doi.org/10.1016/S0303-7207(03)00123-0 (7) "Direct Determination of Product Radical Structure Reveals the Radical Rearrangement Pathway in a Coenzyme B12-Dependent Enzyme" by K. Warncke and J. M. Canfield Journal of the American Chemical Society vol.126 no.19 pp.5930-5931 May 19 2004 http://dx.doi.org/10.1021/ja031569d (8) "Mechanism for Ordered Receptor Binding by Human Prolactin" by U. Sivaprasad, J. M. Canfield, and C. L. Brooks Biochemistry vol.43 no.43 pp.13755-13765 Nov.2 2004 http://dx.doi.org/10.1021/bi049333p (9) "Active Site Reactant Center Geometry in the CoII-Product Radical Pair State of Coenzyme B12-Dependent Ethanolamine Deaminase Determined by Using Orientation-Selection Electron Spin-Echo Envelope Modulation Spectroscopy" by J. M. Canfield and K. Warncke Journal of Physical Chemistry B vol.109 no.7 pp.3053-3064 Feb.24 2005 http://dx.doi.org/10.1021/jp046167m (10) "Engineering Metal Ion Coordination to Regulate Amyloid Fibril Assembly and Toxicity" by J. Dong, J. M. Canfield, A. K. Mehta, J. E. Shokes, B. Tian, W. S. Childers, J. A. Simmons, Z. Mao, R. A. Scott, K. Warncke, and D. G. Lynn Proceedings of the National Academy of Sciences of the United States of America vol.104 no.33 pp.13313-13318 Aug.14 2007 http://www.pnas.org/cgi/doi/10.1073/pnas.0702669104 (11) "Critical Role of Arginine 160 of the EutB Protein Subunit for Active Site Structure and Radical Catalysis in Coenyzme B12-Dependent Ethanolamine Ammonia-Lyase" by Li Sun, O. A. Groover, J. M. Canfield, and K. Warncke Biochemistry vol.47 no.20 pp.5523-5535 Apr.30 2008 http://dx.doi.org/10.1021/bi702366e Thesis Work: P. G. Debrunner (Physics) and R. L. Belford (Chemistry), Advisors: Approaching Magnetic Field Effects in Biology using the Radical Pair Mechanism The overall goal of my Ph.D. thesis work was to explain any of the reported magnetic field effects in biology (magnetic orientation of many species and/or health effects, such as cancer, due to man-made electromagnetic fields) using the radical pair mechanism, a quantum mechanical mechanism known for over 20 years that lets singlet to triplet yields of radical pair reactions (in solid, liquid, or gas phases) depend on applied magnetic fields. This goal seems reasonable considering the known roles of many biological free radicals in cancer, disease, aging, development, and cellular signaling, the constant reminders in the media to take anti-oxidant vitamins to protect against dangerous free radicals, and the success of the radical pair mechanism in explaining magnetic field effects in photosynthetic reaction centers. To approach the above goal, I developed and programmed several computational methods for calculating singlet to triplet yields for radical pair reactions in combinations of steady and oscillating fields (some of these algorithms are more versatile or efficient while others give more insight, and all serve as cross-checks on each other) and used these tools to explore and explain a number of interesting phenomena such as yields sensitive to the magnitude and orientation of earth-strength (0.5 G) steady fields as well as the magnitude, orientation, and frequency of very weak (7 mG or less) oscillating fields. In particular, I studied such effects in coenzyme B12 systems, systems long studied by EPR (the chief method for determining the spin Hamiltonians, spin relaxation rates, and other parameters needed for calculations) in which organometallic cobalt-carbon bonds are cleaved homolytically to form radical pairs used by a number of enzymes including methyl malonyl CoA mutase, which controls the metabolism of certain fatty acids in mammals. I also attended a number of conferences related to this interdisciplinary field where I interacted with people from diverse backgrounds ranging from biology to chemistry to physics. Finally, since this project was not the focus of the research groups in which I worked, it required a great deal of self-motivation and independence. Potential Applications of Ph.D. Thesis Work: (a) Free radical polymerizations/chain reactions often have radical pair reactions in initiation/termination steps and so should allow magnetic field control of product ratios, polymer lengths, etc. (b) Electron-hole pairs & excitons, the heart of many semiconductor devices (such as LED's, photoconductors, etc.), often act like radical pairs and so should allow magnetic field control of electron-hole production/recombination, semiconductor noise, etc. (c) Transition metal catalysts often act like free radicals due to their paramagnetic unpaired d-orbital electrons and so may allow magnetic field control via the radical pair mechanism. (d) UV photodegradation, hi-temperature thermolysis, radiolysis, photolysis, etc. can all lead to radical pair formation via homolytic cleavage of covalent bonds. Thus the chance to recombine (effective cleavage rate) may be sensitive to applied magnetic fields. (e) Photochemistry often involves singlet/triplet excited states, and the intersystem crossing rates of forbidden transitions can depend on magnetic fields. This should allow control of fluorescence, phosphorescence, and emission wavelengths/rates via magnetic fields. (f) The theory behind the radical pair mechanism (quantum mechanics, spin Hamiltonians, spin relaxation, chemical kinetics, thermodynamics, statistical mechanics, Schrodinger and Liouville equations, etc.) is very similar to that for other magnetic resonance phenomena (EPR/ESR,NMR,CIDNP,CIDEP,ODMR,RYDMR,MARY,ESEEM,ENDOR,NOESY,MQR (multiple quantum resonances),etc.), allowing its use in many fields. Postdoctoral Work: C. L. Brooks (Biochemistry, Ohio State University), Advisor: Protein expression & synthesis, site-directed mutagenesis, receptor binding kinetics, & molecular modeling (in Quanta, HyperChem, & Sigma) of the 4-helix bundle proteins prolactin & growth hormone. K. Warncke (Physics, Emory University), Advisor: X- & Q-band EPR/ESR, 2- & 3-pulse ESEEM, ESE-EPR, & Q-band ENDOR experiments & simulations, some for structural & kinetic studies of radical intermediates in the coenzyme B12-dependent enzyme ethanolamine deaminase, & others for structural studies of copper-binding to beta-amyloid complexes. Conferences Attended: Gordon Conference on Bioelectrochemistry: Mechanisms of Electric and Magnetic Field Reception in Animals Tilton, New Hampshire, July 20-24, 1992 Second International Symposium on Magnetic Field and Spin Effects in Chemistry and Related Phenomena University of Konstanz, Konstanz, Germany, July 26-31, 1992 Fifth Annual Cell & Molecular Biology & Molecular Biophysics Research Symposium Beckman Institute, UIUC, September 19, 1992 Third International Symposium on Magnetic Field and Spin Effects in Chemistry and Related Phenomena sponsored by the University of Chicago and Argonne National Laboratory September 25-30, 1994 Beyond Protein Structure: The Pfizer Inc.- Beckman Institute Symposium Beckman Institute, UIUC, June 6-9, 1996 Gordon Conference on Bioelectrochemistry: Molecular and Cellular Biophysics of the Alteration of Biochemical Reactions and Transport by Electric and Magnetic Fields Salve Regina University, Newport, Rhode Island, July 21-26, 1996 Sixth International Symposium on Magnetic Field and Spin Effects in Chemistry and Related Phenomena Emmetten, Switzerland, August 21-26, 1999 Cambridge Healthtech Institute's Protein Structure Conference Washington, D.C., November 15-16, 1999 31st Southeastern Magnetic Resonance Conference (SEMRC) University of Florida, Gainesville, FL, October 26-28, 2001 32nd Southeastern Magnetic Resonance Conference (SEMRC) North Carolina State University, Raleigh, NC, October 25-27, 2002 26th International Electron Paramagnetic Resonance Symposium 45th Rocky Mountain Conference on Analytical Chemistry Denver, Colorado, July 27-31, 2003 8th International Symposium on Spin and Magnetic Field Effects in Chemistry and Related Phenomena University of North Carolina, Chapel Hill, NC, September 21-26, 2003 MathWorks Seminar: Using MATLAB In The Life Sciences Emory University, Atlanta, GA, April 6, 2004 Interface of Computers with Chemistry, Physics, Biology & Materials: Methods & Applications Emerson Center for Scientific Computation, Emory University, May 1, 2004 Computational and Mathematical Modeling in Biological Sciences Emerson Center for Scientific Computation, Emory University, May 4, 2005 34th Southeastern Magnetic Resonance Conference (SEMRC) Emory University, Atlanta, GA, November 11-13, 2005 Computational and Mathematical Modeling in Large Systems: From Proteins to Cells Emerson Center for Scientific Computation, Emory University, April 3, 2006 35th Southeastern Magnetic Resonance Conference (SEMRC) University of Florida, Gainesville, FL, November 3-5, 2006 Systems Biology: Molecules to Populations Emerson Center for Scientific Computation, Emory University, April 6, 2007 Talks Given: "Magnetic Field Effects on Coenzyme B12 Radical Pair Reactions" Theoretical Biophysics Group, Beckman Institute, UIUC, Spring 1992 Illinois EPR Research Center, College of Medicine, UIUC, Spring 1993 "Introduction to CIDNP and CIDEP Effects" Illinois EPR Research Center, College of Medicine, UIUC, Fall 1993 "Approaching Magnetic Field Effects in Biology using the Radical Pair Mechanism" Illinois EPR Research Center, College of Medicine, UIUC, Fall 1994 Grissom Group, Chemistry Department, University of Utah, Summer 1995 Guest Lecture for a Discovery Course on Cancer, UIUC, Spring 1996 Radiation and Photochemistry Group, Argonne National Laboratory, Summer 1996 Radiation Biophysics and Bioengineering in Oncology Group, UIUC, Spring 1997 Illinois EPR Research Center, College of Medicine, UIUC, Spring 1997 Marsh Group, Chemistry Department, University of Michigan, Summer 1998 "Calculations of Earth-Strength Steady and Oscillating Magnetic Field Effects in Coenzyme B12 Radical Pair Systems" Illinois EPR Research Center, College of Medicine, UIUC, Fall 1995 Warncke Group, Physics Department, Emory University, Spring 2000 "Geometry of Reactant Centers in the CoII-Substrate Radical Pair State of Coenzyme B12-Dependent Ethanolamine Deaminase Determined by using 2D-ESEEM Spectroscopy", SEMRC 2001, University of Florida, Fall 2001 "Density Functional Theory: In Pursuit of the 'Divine' Functional" Molecular Biophysics Group, Physics Department, Emory University, Spring 2006 "Magnetic Isotope Effect of Magnesium in Phosphoglycerate Kinase Phosphorylation" Molecular Biophysics Group, Physics Department, Emory University, Spring 2007 Hobbies and Other Interests: Computer programming (games, animation, 3-D display techniques. Have made psychological test analysis programs for my father), drawing, Super 8 movies (animation), jogging, reading. Also interested in computer learning, artificial intelligence, and automation as well as atmospheric, solar, and geological physics. Have taken many life science courses including physiology, organic & biochemistry, computational neuroscience, endocrinology, neural development, etc. References and Transcripts: Available on request